RESEARCH ARTICLE


A New-Generation Method for Quick and Owren PT



Juha Horsti1, *, Helena Uppa 1, Juhani A. Vilpo 2
1 Tampere University Hospital, Centre for Laboratory Medicine, P.O. Box 2000, FIN-33521 Tampere, Finland; University of Tampere, FIN-33521 Tampere, Finland
2 Department of Clinical Chemistry, Centre for Laboratory Medicine, Tampere University Hospital and University of Tampere


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© 2008 Horsti et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Tampere University Hospital, Centre for Laboratory Medicine, P.O. Box 2000, FIN-33521 Tampere, Finland; Tel: +358 3 331 75290; Fax: +358 3 75554; E-mail: juha.horsti@pshp.fi


Abstract

Oral anticoagulant therapy (OAT) calls for continuous control by prothrombin time (PT) test, as the therapeutic range in INR units is very narrow. Warfarin (or coumarin) inhibits coagulation factor synthesis in the liver, but at the same time inactive coagulation factors are formed. The aim here was to measure "active coagulation factors" and inhibition in calibrator kits and patient plasmas by a new method for Quick and Owren PT.

Four calibration kits and 200 plasma samples obtained from OAT patients were assessed using Quick and Owren PT for INRTot (active coagulation factors + inhibition) and INRAcf (only active coagulation factors). Conspicuous variation in inhibition was noted between the four calibration kits. The new- generation PT method develops anticoagulation therapy based on active coagulation factors in vivo and improves INR result harmonization for Quick and Owren PT reagents. This new approach improves Quick PT reliability.

Keywords: PT, prothrombin time, oral anticoagulant therapy.